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Effect of clofibric acid on the molecular species composition of diacyl glycerophosphocholine of rat liver microsomes
Author(s) -
Nakagawa Yasuhito,
Waku Keizo,
Hirose Akihiko,
Kawashima Yoichi,
Kozuka Hiroshi
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537212
Subject(s) - clofibric acid , microsome , glycerophospholipids , composition (language) , microsoma , chemistry , clofibrate , clinical chemistry , biochemistry , enzyme , chromatography , biology , phospholipid , linguistics , philosophy , membrane
The effect of administering p ‐chlorophenoxyisobutyric (clofibric) acid to rats on the molecular species composition of diacyl‐glycerophosphocholine (GPC) of rat liver microsomes was studied. Microsomal choline glycerophospholipids were converted to 1,2‐diradyl‐3‐acetylglycerol and were separated into molecular species by reverse‐phase high performance liquid chromatography. Diacyl‐GPC consisted of 17 different molecular species. The predominant species were arachidonoyl derivatives, such as 18∶0–20∶4 (22.2% of the total) and 16∶0–20∶4 (22.0%). Administration of clofibric acid to rats caused a marked increase in 16∶0–18∶1 species of diacyl‐GPC from 8% to 30%, making these the predominant species of diacyl‐GPC in clofibric acid‐fed rats. Also, a significant decrease (50% of controls) in 18∶0–18∶2 and 18∶0–20∶4 species was observed, whereas the decrease in molecular species containing 16∶0 at the 1‐position such as 16∶0–18∶2 and 16∶0–20∶4 was small (approximately 85% of control). The results show that clofibric acid caused marked changes in the molecular species composition of diacyl‐GPC. The participation of 1‐acyl‐GPC acyltransferase and stearoyl‐CoA desaturase in the regulation of the molecular species composition of diacyl‐GPC is discussed.