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Effect of 4,7,10,13,16,19‐docosahexaenoic acid on triglyceride accumulation and secretion in rat hepatocytes in culture
Author(s) -
Martin L. J.,
Reaidi G. B.,
Gavino G. R.,
Gavino V. C.
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537202
Subject(s) - docosahexaenoic acid , triglyceride , secretion , clinical chemistry , medicine , extracellular , intracellular , oleic acid , reuptake , endocrinology , chemistry , fatty acid , lipidology , hepatocyte , biochemistry , biology , cholesterol , polyunsaturated fatty acid , in vitro , serotonin , receptor
We have investigated the effect of oleic (18∶1) and 4,7,10,13,16,19‐docosahexaenoic (22∶6ω3) acids on triglyceride (TG) accumulation, secretion and reuptake in rat hepatocytes in culture. We also calculated the percentage of total TG, TG‐esterified 18∶1 and TG‐esterified 22∶6ω3 that were secreted relative to the total accumulation (intra+extracellular TG). Both fatty acids were incorporated mainly in the intracellular TG fraction. Treatment with 18∶1 but not with 22∶6ω3 increased the quantity of TG secreted into the culture medium relative to controls. Treatment with 22∶6ω3 caused a greater accumulation of intracellular TG than 18∶1. This arises in part from the preferential retention of 22∶6ω3‐enriched TG by the hepatocytes. At 24 hr, there was no longer any difference in the net secretion of TG by 18∶1 and 22∶6ω3‐treated cells, which may be a consequence of the reuptake of TG‐esterified 18∶1. There was no reuptake of TG‐esterified 22∶6ω3. We conclude that inhibition of hepatocyte TG synthesis is not obligatory for 22∶6ω3‐induced diminution of TG secretion.