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A rapid method for the preparation of ganglioside G lac 2 (GD3)
Author(s) -
Jennemann Richard,
Wiegandt Herbert
Publication year - 1994
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537191
Subject(s) - ganglioside , chromatography , chemistry , behenic acid , methanol , lipidology , sialic acid , ceramide , ethanol , clinical chemistry , fatty acid , bovine milk , biochemistry , organic chemistry , palmitic acid , apoptosis
A method is described for the preparation of ganglioside G lac 2 [(II 3 (NeuAc) 2 ‐LacCer, GD3] from cream of bovine milk using liquid‐phase extraction with methanol or ethanol followed by anion exchange chromatography. The method is rapid and inexpensive; 1 kg cream, centrifuged from 14–15 L of bovine milk, yields approximately 70 mg of pure ganglioside G lac 2. The sialic acid constituent of ganglioside G lac 2 isolated from bovine milk cream consists solely of the N ‐acetylneuraminic acid derivative. The major components of its ceramide consist of octadecasphing‐4‐enine and the 22∶0 (behenic acid) and 23∶0 fatty acids.