Premium
Meningioma phospholipid profiles measured by 31 P nuclear magnetic resonance spectroscopy
Author(s) -
Seijo Luis,
Merchant Thomas E.,
Ven Leo T. M.,
Minsky Bruce D.,
Glonek Thomas
Publication year - 1994
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537190
Subject(s) - plasmalogen , phospholipid , phosphatidylethanolamine , phosphatidylcholine , chemistry , sphingomyelin , phosphatidylserine , phosphatidylinositol , ethanolamine , phosphatidic acid , phosphatidylglycerol , nuclear magnetic resonance spectroscopy , biochemistry , nuclear magnetic resonance , chromatography , membrane , stereochemistry , physics , kinase
Fourteen cases of intracranial meningioma were characterized after chloroform/methanol extraction by 31 P nuclear magnetic resonance (NMR) spectroscopy at 202.4 MHz. Each phospholipid class detected in the extracts was identified and quantitated in terms of its molar percentage relative to the total phospholipids measured. The following phospholipids were assayed by 31 P NMR: phosphatidylglycerol, phosphatidic acid, diphosphatidylglycerol, ethanolamine plasmalogen, phosphatidylethanolamine (PE), lysophosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylcholine (LPC), phosphatidylinositol (PI), sphingosylphosphorylcholine and phosphatidylcholine. In addition, two unidentified phospholipids were detected with resonances at 0.13 and −0.78 ppm, respectively. Three distinct types of spectra were obtained on the extracts and grouped accordingly for comparison purposes. Type 1 tumors showed unusual 31 P NMR profiles with low levels of PE and PI and elevated levels of LPC; type 2 tumors were characterized by low levels of the ethanolamine phospholipids and near equivalent levels of PI and LPC. The spectra of type 1 and type 2 tumors were characteristic of degenerative cells that lacked membrane permeability associated with loss of ethanolamine plasmalogen in the presence of significant phospholipid turnover. Meningiomas belonging to the third spectral type showed characteristics similar to those of normal tissues with normal levels of PE and ethanolamine plasmalogen, as well as very low levels of LPC relative to PI. Type 3 tumors lacked the characteristic signs of degeneration noted in type 1 and type 2 tumors. The data corroborate and augment in vivo spectroscopic findings reported earlier and demonstrate the value of 31 P NMR spectroscopic phospholipid analysis on lipid extracts for the characterization of meningiomas.