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Reevaluation of the neutral lipids of Tilletia controversa and Tilletia tritici
Author(s) -
Beattie S. E.,
Stafford A. E.,
King A. D.
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537129
Subject(s) - glyceride , biology , extraction (chemistry) , chromatography , thin layer chromatography , biochemistry , chemistry , fatty acid
Differential scanning calorimetry of whole teliospores and lipid extracts of Tilletia controversa Kühn and T. tritica Tul. indicated that the lipid composition of teliospores was different than earlier reported. An exothermic peak at −40 to −45°C and an endotherm at −25 to −15°C indicated that the majority of lipids were triacylglycerols (TAG). Hot isopropanol was used to inactivate lipases during lipid extraction. Thin‐layer chromatographic analysis of extracted lipids showed that free fatty acids (FFA) were not present in great quantities unless water was present during lipid extraction. As measured by gas chromatography. FFA accounted for 1–5% of the lipid content in teliospores of Tilletia spp. The TAG content of teliospores was 60–80% of total lipids.