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Phospholipid and fatty acid compositions of Rhizobium leguminosarum biovar trifolii ANU843 in relation to flavone‐activated pSym nod gene expression
Author(s) -
Orgambide Guy G.,
Huang ZhiHeng,
Gage Douglas A.,
Dazzo Frank B.
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537117
Subject(s) - phospholipid , fatty acid , biochemistry , phosphatidylethanolamine , phosphatidylglycerol , rhizobium leguminosarum , chemistry , phosphatidylcholine , unsaturated fatty acid , biology , chromatography , bacteria , rhizobiaceae , symbiosis , membrane , genetics
Abstract The phospholipid and associated fatty acid compositions of the bacterial symbiont of clover, Rhizobium leguminosarum biovar trifolii wild‐type ANU843, was analyzed by two‐dimensional silica thin‐layer chromatography, fast atom bombardment‐mass spectrometry, flame‐ionization detection gas‐liquid chromatography and combined gas‐liquid chromatography/mass spectrometry. The phospholipid composition included phosphatidylethanolamine (15%), N ‐methylphosphatidylethanolamine (47%), N,N ‐dimethylphosphatidylethanolamine (9%), phosphatidylglycerol (19%), cardiolipin (5%) and phosphatidylcholine (2%). Fatty acid composition included predominantly cis ‐11‐octadecenoic acid, lower leves of cis ‐9‐hexadecenoic acid, hexadecanoic acid, 11‐methyl‐11‐octadecenoic acid, octadecanoic acid, 11,12‐methyleneoctadecanoic acid, eicosanoic acid and traces of branched, and di‐ and triunsaturated fatty acids. The influence of expression of the “nodulation” genes encoding symbiotic functions on the composition of these membrane lipids was examined in wild‐type cells grown with or without the flavone inducer, 4′,7‐dihydroxyflavone and in mutated cells lacking the entire symbiotic plasmid where these genes reside, or containing single transposon insertions in selected nodulation genes. No significant changes in phospholipid or associated fatty acid compositions were detected by the above methods of analysis.