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Effects of high corn oil diet on preneoplastic murine colons: Prostanoid production and lipid composition
Author(s) -
Robblee Nancy M.,
Bird Ranjana P.
Publication year - 1994
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537093
Subject(s) - corn oil , prostanoid , lipidology , intestinal mucosa , biology , prostaglandin , medicine , clinical chemistry , dimethylhydrazine , carcinogen , saline , endocrinology , food science , chemistry , biochemistry , colorectal cancer , cancer
In the present study, the effect of normal (5% by wt) and high (23.5% by wt) corn oil diets on prostanoid production and on the lipid composition of preneoplastic colonic epithelium was investigated. CF 1 mice (female, 3–4‐weeks‐old) were fed a normal corn oil diet ad libitum and were treated with the colon carcinogen 1,2‐dimethylhydrazine (DMH, 20 mg/kg/wk) or saline (control) for 24 wk. At this stage, all animals received the AIN‐76 diet (normal corn oil) ad libitum . Following the last injection, half of the animals from each treatment group were randomly allocated to a high corn oil diet for 5 to 10 wk, whereas the remaining animals continued on the normal corn oil diet. After 5 wk of feeding, the colonic mucosa of carcinogentreated animals had a higher level of bicyclic prostaglandin E 2 (PGE 2 ) than had the animals in the control groups; prostanoid synthesis in the colonic mucosa of control animals was unaffected by the high corn oil diet. Preneoplastic colonic mucosa of animals fed the high corn oil diet had a significantly higher level of PGE 2 than corresponding control colonic mucosa. The 6‐keto‐prostaglandin F 1α /thromboxane B 2 ratio was significantly lower in the DMH‐treated groups than in the control groups, and was unaffected by dietary treatments. After 10 wk of feeding a particular diet, the differences in the fatty acid composition between the control and DMH‐treated groups were minor. Our findings demonstrate that the preneoplastic colonic epithelium differs from that of normal epithelium with respect to prostanoid synthesis.