z-logo
Premium
Regulation of rat liver microsomal cholesterol ester hydrolase by reversible phosphorylation
Author(s) -
Martínez María J.,
Hernández María L.,
Lacort Mercedes,
Ochoa Begoña
Publication year - 1994
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537084
Subject(s) - calmodulin , chemistry , trifluoperazine , phosphatase , biochemistry , dephosphorylation , hydrolase , protein kinase a , phosphorylation , microsome , okadaic acid , alkaline phosphatase , enzyme
The regulation of neutral cholesterol ester hydrolase activity by changes in its phosphorylation state was studied in rat liver microsomes. Treatment with cAMP‐dependent protein kinase resulted in increased enzyme activity, which was further enhanced by the addition of cAMP and MgATP. Consistent activations were also achieved with MgCl 2 and MgATP, the magnesium effect being abolished by ethylenediaminetetraacetic acid and adenosine triphosphate. Cholesterol ester hydrolase was activated twofold by free calcium and Ca 2+ /calmodulin; this latter effect was blocked by the chelator ethyleneglycol‐ bis (β‐aminoethyl ether) N,N,N′,N′ ‐tetraacetic acid and the calmodulin antagonist trifluoperazine. The phosphatase inhibitors pyrophosphate and glycerophosphate led to marked and dose‐dependent increases in esterase activity, whereas okadaic acid elicited no effect. Furthermore, pyrophosphate and okadaic acid did not change the increases in enzyme activity promoted by Ca 2+ , Ca 2+ /calmodulin, Mg 2+ and MgATP. Cholesterol ester hydrolase was inactivated in a concentration‐dependent manner by nonspecific alkaline phosphatases. In cAMP‐dependent protein kinase/cAMP‐ or Ca 2+ /calmodulin‐activated microsomes, a time‐dependent loss of activation in cholesteryl oleate hydrolysis was caused by alkaline phosphatase. These findings suggest that microsomal cholesterol ester hydrolase is activated through cAMP and Ca 2+ /calmodulin phosphorylation, whereas enzyme deactivation is dependent on phosphatase action.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here