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Synthesis of arachidonic acid metabolites by syrian hamster platelets and peritoneal cells
Author(s) -
Surette Marc E.,
Whelan Jay,
Lu G. P.,
Kinsella John E.
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537082
Subject(s) - zymosan , arachidonic acid , thromboxane b2 , leukotriene b4 , thromboxane , chemistry , hamster , platelet , nordihydroguaiaretic acid , in vivo , eicosanoid , leukotriene c4 , peritoneal cavity , stimulation , prostaglandin , prostaglandin e2 , endocrinology , pharmacology , biochemistry , leukotriene , medicine , in vitro , biology , inflammation , microbiology and biotechnology , anatomy , asthma , enzyme
In this study, the metabolism of arachidonic acid by hamster platelets and peritoneal macrophages was assessed. Peritoneal macrophages stimulated in vitro with the calcium ionophore A23187 or stimulated in vivo by intraperitoneal injections of opsonized zymosan produced prostaglandin E 2 , thromboxane B 2 (TxB 2 ) and 6‐keto‐prostaglandin F 1α , as determined by radioimmuno assays. Leukotriene B 4 (LTB 4 ), and 11‐ and 15‐hydroxyeicosatetraenoic acids (HETE), which were identified by reverse‐phase highperformance liquid chromatography coupled with diode array detection, were produced by peritoneal cells stimulated in vitro with A23187 but were not found in the peritoneal exudate following in vivo stimulation with opsonized zymosan. Synthesis of 11‐ and 15‐HETE, but not LTB 4 , was inhibited by 1 μM indomethacin but not by 10 μM nordihydroguaiaretic acid, which did inhibit LTB 4 synthesis. Washed hamster platelets were prepared and shown to synthesize TxB 2 , 12‐HETE and 12‐hydroxyheptadecatrienoic acid following stimulation with thrombin. This paper is the first to report on eicosanoid metabolism in tissues related to atherosclerosis, thrombosis and inflammation in hamsters.