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Effects of trans fatty acids on lipid accumulation in 3T3‐L1 cells
Author(s) -
Panigrahi Kalpana,
Sampugna Joseph
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537072
Subject(s) - adipose tissue , adipocyte , fatty acid , clinical chemistry , chemistry , lipidology , biochemistry , lipid metabolism , lipid droplet , 3t3 l1 , in vivo , biology , microbiology and biotechnology
Previous work had shown that dietary trans fatty acids ( t FA) resulted in decreased fat deposition in adipose tissue. This study was conducted to see if t FA influence lipid accumulation in Swiss mouse fibroblast 3T3‐L1 cells, which are widely used as an adipocyte model. Cells were cultured in the presence of experimental or control growth media supplemented with fatty acids complexed to bovine serum albumin. Fatty acid compositions of experimental and control growth media were similar except that the octadecenoates in the control growth media were cis fatty acids, whereas those in the experimental media contained both cis and trans fatty acids. Cell‐conditioned media and cellular lipids at the preadipocyte and differentiating adipocyte stages were analyzed. At both stages of development, less fat accumulated, in cells cultured in the presence of t FA, due primarily to a decrease in the nonpolar lipid content of cells exposed to t FA, and linoleate to arachidonate ratios were higher in cells supplemented with t FA. Calculations comparing sums of saturated and monounsaturated fatty acids in cells at the differentiating adipocyte stage suggested that t FA may have replaced monoun‐saturated fatty acids in the nonpolar lipid fraction and saturated fatty acids in the polar lipid fraction. The results of these studies are in good agreement with the in vivo effects of t FA seen in previous work with mouse adipose tissue. It was concluded that the 3T3‐L1 in vitro model is an appropriate system for further studies of t FA and lipid metabolism in adipose tissue.