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Lipids of selected molds grown for production of n−3 and n−6 polyunsaturated fatty acids
Author(s) -
Kendrick Andrew,
Ratledge Colin
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537052
Subject(s) - polyunsaturated fatty acid , docosahexaenoic acid , arachidonic acid , biochemistry , fatty acid , atp citrate lyase , eicosapentaenoic acid , food science , degree of unsaturation , chemistry , phospholipid , biology , enzyme , chromatography , citrate synthase , membrane
The lipid classes and component fatty acids of seven fungi were examined. Three marine fungi, Thraustochytrium aureum, Thraustochytrium roseum and Schizochytrium aggregatum (grown at 30, 25 and 25°C, respectively), produced less than 10% lipid but contained docosahexaenoic acid (DHA) up to 30% and eicosapentaenoic acid (EPA) up to 11% of the total fatty acids. Mortierella alpinapeyron produced 38% oil containing solely n‐6 polyunsaturated fatty acids (PUFA) with arachidonic acid (AA) at 11% of the total fatty acids. Conidiobolus nanodes and Entomorphthora exitalis produced 25% oil and contained both n−3 and n−6 PUFA, with AA at 16% and 18%, respectively. Saprolegnia parasitica produced 10% oil and contained AA and EPA, respectively, at 19% and 18%. The triacylglycerol fraction always represented the major component at between 44% and 68% of the total lipid. Each fungus, except T. aureum , had the greatest degree of fatty acid unsaturation in the phospholipid fraction. The triacylglycerol fraction of T. aureum was the most unsaturated with DHA representing 29% (w/w) of all fatty acids present. The presence of the enzyme ATP:citrate lyase correlated with the ability of molds to accumulate more than 10% (w/w) lipid when the fungi were grown in nitrogen‐limiting media. In those molds that failed to accumulate more than 10% lipid, the enzyme was absent.