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Compartmental study of rat renal phospholipid metabolism
Author(s) -
SterinSpeziale Norma,
Kahane Veronica L.,
Setton Clara Patricia,
Fernandez Maria del Carmen,
Speziale Emir H.
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537051
Subject(s) - phosphatidylethanolamine , phospholipid , renal papilla , phosphatidylserine , phosphatidylcholine , cortex (anatomy) , chemistry , phosphatidylinositol , biochemistry , palmitic acid , sphingomyelin , major duodenal papilla , renal cortex , metabolism , biology , endocrinology , membrane , fatty acid , kidney , anatomy , kinase , neuroscience
Phospholipid content and metabolism were studied in rat renal papillary, medullary and cortical slices. The highest concentration of phospholipids was found in cortex and the lowest in papilla samples (ratio cortex/medulla, 1.3; cortex/papilla, 3.7). The profile of the various phospholipids was different depending on the zone. The most important difference was the relative concentrations of sphingomyelin (Cer P Cho) and phosphatidylinositol (PtdIns) with ratios for PtdIns/Cer P Cho of 5.0, 3.3 and 2.5 in papilla, medulla, and cortex, respectively. In the three zones, PtdIns showed the highest specific activity for [2‐ 14 C]glycerol and [1‐ 14 C]arachidonic acid incorporation. By contrast, a higher amount of [1‐ 14 C]palmitic acid was incorporated into phosphatidylcholine than into any other phospholipid. The various radioactive precursors were only poorly incorporated into phosphatidylethanolamine. No radioactivity was associated with phosphatidylserine. The papilla possesses the most active phospholipid metabolism of all the pathways studied.