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Inhibition of soybean lipoxygenase‐1 by chain‐breaking antioxidants
Author(s) -
Maccarrone Mauro,
Veldink Gerrit A.,
Vliegenthart Johannes F. G.,
Agrò Alessandro Finazzi
Publication year - 1995
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537041
Subject(s) - trolox , lipoxygenase , chemistry , ascorbic acid , antioxidant , radical , lipid peroxidation , enzyme , biochemistry , food science , antioxidant capacity
The aim of this investigation was to determine whether chain‐breaking antioxidants able to prevent lipid peroxidation can inhibit lipoxygenase‐1 (EC 1.13.11.12). Therefore, the effects of ascorbic acid, 6‐palmitoylascorbic acid and trolox on the enzyme activity were analyzed by means of Lineweaver‐Burk double reciprocal plots and Yoshino's graphical method. The effect of these compounds on the formation of free radicals during lipoxygenase‐1 reaction was investigated as well, by monitoring the enzymic formation of oxodienes. We present evidence that the chain‐breaking antioxidants ascorbic acid, 6‐palmitoylascorbic acid and trolox inhibit soybean lipoxygenase‐1 in the micromolar concentration range (Ki 27, 3 and 18 μM, respectively). The inhibition is competitive, complete and reversible. All three compounds trap the free radicals formed during the lipoxygenase‐catalyzed reaction, which might substantially contribute to their inhibitory ability. These findings can have physiological significance in the light of the lipoxygenase involvement in biomembrane remodelling.