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A thermospray mass spectrometric assay for Fe‐induced 4‐hydroxynonenal in tissues
Author(s) -
Blanchflower W. John,
Walsh Dominic M.,
Kennedy Seamus,
Kennedy D. Glenn
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536650
Subject(s) - thermospray , lipidology , clinical chemistry , 4 hydroxynonenal , chemistry , mass spectrometry , chromatography , biochemistry , tandem mass spectrometry , selected reaction monitoring , lipid peroxidation , oxidative stress
A method is presented for the determination of 4‐hydroxy‐nonenal (HNE) in tissue homogenates following in vitro lipid peroxidation induced by iron (Fe ++ ). NHE is measured as the pentafluorobenzyl oxime derivative using liquid chromatography thermospray mass spectrometry. In vitro metabolism of HNE via the glutathione/glutathione‐ S ‐transferase pathway was inhibited using iodoacetic and iodobenzoic acids. The assay has been used as an indicator of the peroxidizability of tissue samples from animals both adequate in and depleted of α‐tocopherol. The concentrations of HNE produced in tissues taken from animals depleted of α‐tocopherol were found to be up to 8 times higher than those taken from animals supplemented with α‐tocopherol.