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Analysis of low erucic acid turnip rapeseed oil ( Brassica campestris ) by negative ion chemical ionization tandem mass spectrometry. A method giving information on the fatty acid composition in positions sn ‐2 and sn ‐1/3 of triacylglycerols
Author(s) -
Kallio Heikki,
Currie Graeme
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536641
Subject(s) - erucic acid , chemistry , rapeseed , mass spectrometry , brassica , chromatography , chemical ionization , fatty acid , linolenic acid , linoleic acid , tandem mass spectrometry , oleic acid , food science , organic chemistry , biochemistry , ionization , ion , botany , biology
A tandem mass spectrometric method is described for the rapid analysis of fatty acid combinations in mixtures of triacylglycerols. Triacylglycerols were introduced into a triple quadrupole mass spectrometer via a direct exposure probe and deprotonated using ammonia negative ion chemical ionization. Collisionally activated spectra were obtained and the resulting fragments used to identify the fatty acid constituents, and the fatty acids preferentially located at the sn ‐2 position of the triacylglycerols. Fourteen major molecular weight species of purified triacylglycerols of a supercritical fluid extract of low erucic acid turnip rapeseed oil ( Brassica campestris ) were analyzed. The five major combinations of fatty acids comprised two thrids of the total triacylglycerols and contained oleic, linoleic and α‐linolenic acids with linoleic acid favoring the sn ‐2 position.