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The effect of culture medium composition on ether lipid cytotoxic activity
Author(s) -
Diomede Luisa,
Piovani Bianca,
Modest Edward J.,
Salmona Mario
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536638
Subject(s) - fetal bovine serum , chemistry , k562 cells , hl60 , cell culture , cholesterol , composition (language) , clinical chemistry , microbiology and biotechnology , biochemistry , in vitro , biology , linguistics , philosophy , genetics
The effect of a serum‐free medium (TNB‐100), compared to RPMI 1640 containing 10% fetal bovine serum (FBS), on the lipid composition of HL60 and K562 leukemic cells was investigated. The 10% FBS RPMI medium contained approximately three times more phospholipids (PL), about three times more protein and eight times more cholesterol (CHOL) than did the TNB‐100 medium. Cells cultured in TNB‐100 medium, referred to as HL60‐TNB and K562‐TNB cells, were significantly lower in PL and CHOL than 10% FBS RPMI cells, with about a threefold higher PL‐to‐CHOL ratio; however, these cells were significantly higher in protein content. Cells grown in TNB‐100 were also significantly more fluid than 10% FBS RPMI cells and were more sensitive to the fluidizing action of the ether lipid 1‐ O ‐octadecyl‐2‐ O ‐methyl‐ rac ‐glycero‐3‐phosphocholine. The 50% inhibitory dose of the drug was about 50% lower in TNB‐grown cells than in 10% FBS RPMI cells.