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Acylation of lyso platelet‐activating factor by splenocytes of the rainbow trout, Oncorhyncus mykiss
Author(s) -
Pool G. L.,
Samples B.,
Turner M. R.,
Lumb R. H.
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536596
Subject(s) - platelet activating factor , rainbow trout , acylation , glycerophospholipid , phosphocholine , acetylation , biochemistry , chemistry , lyso , arachidonic acid , membrane , phospholipid , biology , phosphatidylcholine , endocrinology , fish <actinopterygii> , enzyme , gene , catalysis , scintillator , engineering , detector , electrical engineering , fishery
In mammalian systems, platelet‐activating factor, 1‐ O ‐alkyl‐2‐acetyl‐ sn ‐glycero‐3‐phosphocholine, (PAF) is rapidly inactivated by a deacetylation/reacylation system that produces 1‐ O ‐alkyl‐2‐acyl‐ sn ‐glycero‐3‐phosphocholine which is highly enriched in arachidonic acid. There is some evidence that n−3 fatty acids may have an impact on this system in humans but the nature of this impact is unclear. In rainbow trout, n−3 fatty acids are known to be essential dietary components which are derived through the food chain. Substantial quantities of n−3 fatty acids are found in trout membrane phospholipids. We show here that in sharp contrast to mammalian cells, trout cells acylate lyso platelet‐activating factor, alkyl‐GPC, 1‐ O ‐alkyl‐2‐lyso‐ sn ‐glycero‐3‐phosphocholine, (lyso‐PAF) with a high degree of specificity for n−3 fatty acids. When [ 3 H]lysoPAF was incubated with these cells, only three molecular species of alkylacylglycerophosphocholine were produced, and 92% contained n−3 fatty acids. Since isolated membranes yielded similar results, it appears that the acylation proceeds via a coenzyme A‐independent transacylase as found in mammalian systems.