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Platelet‐activating factor and granulocyte‐mediated oxidative stress. Strategy for in vivo oxyradical visualization
Author(s) -
Suematsu Makoto,
Tsuchiya Masaharu
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536568
Subject(s) - oxidative stress , in vivo , platelet , lipidology , clinical chemistry , granulocyte , chemistry , platelet activating factor , microbiology and biotechnology , biology , immunology , biochemistry , genetics
Abstract Platelet‐activating factor (PAF) and leukotriene B 4 are potent lipid mediators of endothelium‐granulocyte interaction which results in granulocyte‐dependent increased microvascular permeability. The specific mechanism by which PAF induces granulocyte‐mediated endothelial injury has not been fully investigated. Digital imaging photonic intensified microscopy has revealed that PAF effectively induces granulocyte‐mediated oxidative stress on microvascular beds. The method has made it possible to visualize luminol‐dependent photonic burst released from PAF‐treated microvascular beds in the rat mesentery. The photonic activities clearly corresponded to the localization of sticking granulocytes in post‐capillary venules. By contrast, no significant chemilumigenic response could be detected in the leukotriene B 4 ‐induced activation of endothelium‐granulocyte interaction in vivo . The present findings suggest that oxygen radicals are not prerequisites for granulocyte adhesion in the microcirculation and provide evidence for a dissociation of in vivo granulocyte function between leukotactic and oxidative activation in leukotriene B 4 ‐induced microvascular changes.