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Platelet activating factor‐induced pulmonary accumulation of 111 indium‐oxine labelled neutrophils in anesthetized guinea pigs
Author(s) -
HultkvistBengtsson Ursula,
Anderson Gary P.,
Kings Madeleine,
Morley John
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536549
Subject(s) - chemistry , guinea pig , ethidium bromide , platelet , platelet activating factor , in vitro , acridine orange , microbiology and biotechnology , methionine , endocrinology , medicine , biochemistry , biology , apoptosis , amino acid , dna
The thoracic accumulation of neutrophils labelled with 111 Indium‐oxine in response to infusion of platelet activating factor (PAF, 18 ng/kg/min × 5 min, i.v.) was studied using an automated isotope monitoring system (AIMSplus) in anesthetized guinea‐pigs. Loss of cell associated radioactivity in vitro was less than 1% over 4 hr. Labelled neutrophils maintained their functional capacity (oxidative response to the cell stimulants N‐formyl‐L‐methionine‐L‐leucine‐L‐phenylalanine and phorbol myristate acetate) and >95% viability (ethidium bromide/acridine orange stain) in vitro . Total thoracic radioactivity increased significantly from baseline in response to PAF with a slight tachyphylaxia in the neutrophil‐accumulation after a repeat PAF infusion. The highest ratios of radiolabel (tissue/blood) were found in the spleen >>liver>lung.