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Role of platelet‐activating factor (PAF) in superoxide production by human polymorphonuclear leukocytes
Author(s) -
Takahashi Shuji,
Yoshikawa Toshikazu,
Naito Yuji,
Tanigawa Toru,
Yoshida Norimasa,
Kondo Motoharu
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536537
Subject(s) - superoxide , platelet activating factor , chemistry , zymosan , ionophore , phorbol , platelet , opsonin , biochemistry , reactive oxygen species , calcium , pharmacology , endocrinology , immunology , protein kinase c , in vitro , biology , signal transduction , enzyme , organic chemistry , membrane
The effect of platelet‐activating factor (PAF) in superoxide production by human polymorphonuclear leukocytes (PMN) was studied. Cypridina luciferin analog (CLA) dependent chemiluminescence was used to detect superoxide anion radicals. PAF induced superoxide generation in human PMN in a dose‐dependent manner. Preincubation with a small amount of PAF (5 x 10 −9 M) enhanced PMN superoxide release induced by various stimuli, such as phorbol myristate acetate (PMA), opsonized zymosan (OZ), calcium ionophore (A23187) and N ‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP). The PAF antagonist, CV‐6209, inhibited superoxide production induced by PAF, but not that induced by other stimuli. These findings would indicate that PAF may play an important role at inflammatory reaction sites and that CV‐6209 may inhibit excessive inflammatory reaction.