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Immunoregulatory functions of paf‐acether. VI. Dual effect on human B cell proliferation
Author(s) -
Leprince Corinne,
Vivier Eric,
Treton Dominique,
Galanaud Pierre,
Benveniste Jacques,
Richard Yolande,
Thomas Yolene
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536532
Subject(s) - raji cell , cell growth , b cell , immune system , microbiology and biotechnology , cell , chemistry , cell culture , antibody , phorbol , cytokine , biology , immunology , biochemistry , signal transduction , protein kinase c , genetics
Abstract The role of paf‐acether (paf), a phospholipid cytokine, in the modulation of human B cell function was investigated. Paf, from 1×10 −5 M to 10 −6 M, decreased B cell proliferation induced by both phorbol myristate acetate (PMA) and anti‐IgM antibodies (anti‐IgM Ab). By contrast, 1×10 −7 M to 1×10 −9 M paf enhanced PMA triggered, but not anti‐IgM triggered B cell proliferation. B cell proliferation was modulated between 24 and 72 hr of culture indicating that the effect of paf did not merely reflect a shift in proliferation kinetics. Interestingly, paf also enhanced the spontaneous proliferation of a Burkitt lymphoma‐derived B cell line, Raji, which suggests that paf can directly act on B cells. The modulatory effect of paf on peripheral blood B cells was independent of PMA concentration, yet the effect on Raji cells was dependent upon cell density. The data suggest that paf is a potent modulator of B cell function, and may be involved in the control of humoral immune response.