Premium
Bioactions of 5‐hydroxyicosatetraenoate and its interaction with platelet‐activating factor
Author(s) -
Rossi Adriano G.,
O'Flaherty Joseph T.
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536528
Subject(s) - lipidology , clinical chemistry , factor (programming language) , platelet activating factor , platelet , chemistry , medicine , biochemistry , computer science , programming language
In a variety of stimulated cells, platelet‐activating factor (PAF) and numerous arachidonate derivatives are coproducts that form as a consequence of receptor‐mediated phospholipid mobilization. These lipid co‐products produce a plethora of biological effects in a wide variety of cell systems. Furthermore, they often have a fascinating, although less widely appreciated, interaction. 5‐HETE, at submicromolar concentrations, exerts relatively few direct bioactions. It does, however, potently (16–160 nM) raise cytosolic free calcium [Ca 2+ ] i and augment PAF‐induced responses in human polymorphonuclear neutrophils (PMN) by as much as 100‐ to 1000‐fold. 5‐HETE acts on PMN by a structurally specific, stereospecific and pertussis toxin‐inhibitable mechanism. In addition, PMN exposed to 5‐HETE exhibit homologous but not heterologous desensitization. These findings suggest that 5‐HETE, like PAF, may bind to its own specific plasmalemmal receptors to exert its unique set of bioactions. However, further investigation is required to demonstrate any putative 5‐HETE receptors. Other potential mechanisms of 5‐HETE‐induced bioactions together with the possible effects of 5‐HETE on PAF transduction mechanisms are also discussed.