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A specific, sensitive and high‐capacity immunoassay for PAF
Author(s) -
Baldo Brian A.,
Smal Mary A.,
McCaskill Alistair C.
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536517
Subject(s) - lysophosphatidylcholine , hapten , immunoassay , chemistry , radioimmunoassay , choline , chromatography , phosphatidylcholine , platelet activating factor , ether , clinical chemistry , biochemistry , antibody , phospholipid , medicine , biology , organic chemistry , immunology , membrane
A specific radioimmunoassay for platelet‐activating factor (PAF) sensitive in the range 10–1000 pg (0.02–2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C‐2 of PAF, a requirement for the ether linkage at C‐1 and some tolerance for substituents on the choline nitrogen. No significant cross‐reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.