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Phospholipids in Trypanosoma cruzi : Phosphoinositide composition and turnover
Author(s) -
Racagni G.,
Garcia de Lema M.,
Domenech C. E.,
Machado de Domenech E. E.
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536475
Subject(s) - lipidology , trypanosoma cruzi , clinical chemistry , composition (language) , chemistry , biochemistry , biology , philosophy , linguistics , parasite hosting , world wide web , computer science
The polyphosphoinositides from Trypanosoma cruzi were isolated by preparative thin‐layer chromatography (TLC) and identified. When myo‐[ 3 H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol (lysoPI), phosphatidylinositol 4‐phosphate (PIP) and phosphatidylinositol 4,5‐bisphosphate (PIP 2 ). Short‐term incubation with 32 P i led to higher percentages of incorporation into phosphatidylethanolamine (PE), lysophosphatidylethanolamine (lysoPE) and PI compared to the other glycerophospholipids. The phosphoinositides (PI, PIP and PIP 2 ) contained a larger proportion of unsaturated than saturated fatty acids. High proportions of 18∶2 were found in the three phosphoinositides analyzed, whereas the major saturated fatty acid was 18∶0. Watersoluble inositol phosphates (IP, IP 2 and IP 3 ) were also identified.