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Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase
Author(s) -
Chambon Christophe,
Ladeveze Veronique,
Servouse Madeleine,
Blanchard Laurence,
Javelof Catherine,
Vladescu Barbu,
Karst Francis
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536428
Subject(s) - farnesyl diphosphate synthase , ergosterol , farnesol , biochemistry , yeast , mutant , saccharomyces cerevisiae , enzyme , biology , mevalonate pathway , auxotrophy , mevalonic acid , biosynthesis , chemistry , gene
Yeast mutant strains auxotrophic for ergosterol and blocked in mevalonate diphosphate decarboxylase ( erg19 ) and farnesyl diphosphate (FPP) synthetase ( erg20 ) were isolated. The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol. The isolation of the functional ERG20 gene allowed us to show that farnesyl diphosphate synthetase could be a rate limiting enzyme in ergosterol biosynthesis in yeast.