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Distribution of vitamins A and E in blood and liver of rats depleted of vitamin A or vitamin E
Author(s) -
Omaye Stanley T.,
Chow Faye I.
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536405
Subject(s) - vitamin e , vitamin , lipidology , clinical chemistry , chemistry , medicine , endocrinology , platelet , whole blood , biochemistry , antioxidant
Young Sprague‐Dawley male rats (n=150) were fed a semipurified diet, either without vitamin A (VA), without vitamin E (VE) or supplemented with both vitamins A and E (control). At the end of weeks 0, 1, 3, 5, 6, 7, 8 and 9, groups of rats were anesthetized with methoxyflurane, and blood was collected by cardiac puncture until the rat was exsanguinated. The liver was excised. Whole blood (WB_ from each rat was fractionated into plasma (PLA), leukocytes (LEU), platelets (PLT) and erythrocytes (RBC). Each blood components was extracted with hepatane and livers were extracted with CHCl 3 /CH 3 OH (2∶1, v/v). The extracts were analyzed for VA and VE by high performance liquid chromatography. The relationship among blood components in the loss of VA was PLT=LEU>WB>PLA. The relationship among blood components in the loss of VE was PLA>RBC>WB>LEU‐PLT. VA and VE levels in other blood components decreased precipitously between weeks 0 and 4 in the animals placed on deficient diets. These results and correlation analyses between vitamin contents of blood components and of livers indicate inadequacies for the use of certain blood components as monitors of lipid‐soluble vitamin status in the rat.