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Cholesterol interference in analysis of fatty acid methyl esters
Author(s) -
Hayes Lyle,
Lowry Robert R.,
Tinsley Ian J.
Publication year - 1971
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536378
Subject(s) - fatty acid methyl ester , polyunsaturated fatty acid , chemistry , salmo , fatty acid , trout , clinical chemistry , lipidology , chromatography , gas chromatography , transesterification , biochemistry , organic chemistry , fish <actinopterygii> , biology , methanol , fishery , catalysis , biodiesel
In the course of isolating and analyzing the fatty acid methyl esters (FAME) from steelhead trout ( Salmo gairdneri ) eggs and sac fry, a component was found on gas liquid chromatography to have a retention time longer than any characteristic polyunsaturated fatty acid previously encountered in trout. Subsequent purification and analysis demonstrated this component to be cholesterol. Thus, cholesterol and related compounds can interfere in the analysis of FAME when the methyl esters are prepared by transesterification.