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Leukotriene B 4 stimulation of an early elevation of phosphatidic acid mass in human neutrophils
Author(s) -
Tou Jensie,
Dola Thao
Publication year - 1995
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536294
Subject(s) - lipidology , phosphatidic acid , clinical chemistry , stimulation , chemistry , medicine , endocrinology , biochemistry , phospholipid , membrane
The signal transduction pathway of leukotriene B 4 involves phospholipase D activation in cytochalsin B‐primed neutrophils, but leukotriene B 4 stimulation of increased phosphatidic acid mass in neutrophils has not been demonstrated. Employing the NIH Image program, we have examined the effect of leukotriene B 4 on phosphatidic acid mass in human neutrophils incubated with or without cytochalasin B. Our results show that 0.15 μM leukotriene B 4 without cytochalasin B was capable of increasing phosphatidic acid mass in neutrophils by 2‐fold after 5 s, 2.5‐fold after 1 min, and 2‐fold after 5 min incubation. Leukotriene B 3 , leukotriene B 4 , and leukotriene B 5 were equipotent stimuli for phosphatidic acid mass elevation. Leukotriene B 4 induced phosphatidylethanol formation at the expense of phosphatidic acid in cells preincubated with 0.25–1% ethanol, indicating phospholipase D activation. Cytochalasin B enhanced leukotriene B 4 stimulation of phosphatidic acid mass elevation and phosphatidylethanol formation. There were no measurable changes in 1,2‐diglyceride mass after 5 s, but a 1.7‐fold increase occurred after 1 min and declined thereafter. Leukotriene B 4 stimulation of [ 3 H]glycerol incorporation into phosphatidic acid, diglyceride and phosphatidylinositol was detectable after a 1‐min incubation, suggesting increased de novo synthesis of these lipids. These results suggest that leukotriene B 4 stimulation of phospholipase D activity contributes to part of the early increased phosphatidic acid mass and that combined actions of stimulated phospholipases C and D, and de novo phosphatidic acid synthesis contribute to the total increased phosphatidic acid mass.