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Phospholipid profiling of sediments using phosphorus‐31 nuclear magnetic resonance
Author(s) -
Bardygulan Lidia G.,
Kaster Jerry L.,
Glonek Thomas
Publication year - 1995
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536290
Subject(s) - phospholipid , chemistry , sediment , phosphorus , chloroform , environmental chemistry , phosphate , potassium , nuclear magnetic resonance spectroscopy , chromatography , geology , biochemistry , organic chemistry , membrane , paleontology
A phosphorus‐31 nuclear magnetic resonance method has been developed for the determination of aquatic sediment phospholipid profiles that may be generally applied to all soils and deposits containing viable cellular material. A method of scrubbing chloroform/methanol extracts with potassium acid phosphate overcomes adverse signal broadening from the mineral component, permitting eleven sediment phospholipids to be determined at the quantitative level.