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Rapid determination of double bond configuration and position along the hydrocarbon chain in cyclic fatty acid monomers
Author(s) -
Mossoba M. M.,
Yurawecz M. P.,
Roach J. A. G.,
Lin H. S.,
McDonald R. E.,
Flickinger B. D.,
Perkins E. G.
Publication year - 1994
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536259
Subject(s) - chemistry , double bond , mass spectrometry , gas chromatography , monomer , fourier transform infrared spectroscopy , infrared spectroscopy , chromatography , mass spectrum , gas chromatography–mass spectrometry , electron ionization , fatty acid , organic chemistry , ionization , polymer , physics , quantum mechanics , ion
This study reports the structural elucidation of diunsaturated 5‐ or 6‐membered ring cyclic fatty acid monomers (CFAM) isolated from heated flaxseed oil by complementary gas chromatography (GC)‐mass spectrometry (MS) and GC‐matrix isolation‐Fourier transform infrared spectroscopy (MI‐FTIR). Infrared measurements of CFAM were carried out on methyl ester derivatives as well‐resolved chromatograms were obtained on a polar 100% cyanopropyl polysiloxane capillary GC column. By contrast, electron ionization MS of methyl ester derivatives was of limited value because of double bond migration during the ionization process in the mass spectrometer. This communication reports definitive MS fragmentation patterns that can confirm ring position and double bond position along the fatty acid chain in 1,2‐disubstituted CFAM determined as 2‐alkenyl‐4,4‐dimethyloxazoline derivatives. Double bond configuration ( cis, trans , or conjugated cis,cis ) in CFAM was confirmed by GC‐MI‐FTIR. The presence of CFAM, degradation products found in used frying oils, is a potential source of dietary toxicity.