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Improved resolution of 31 P nuclear magnetic resonance spectra of phospholipids from brain
Author(s) -
Capuani G.,
Aureli T.,
Miccheli A.,
Di Cocco M. E.,
Ramacci M. T.,
Delfini M.
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536154
Subject(s) - resolution (logic) , micelle , nuclear magnetic resonance , spectral line , aqueous solution , chemistry , clinical chemistry , spectral resolution , analytical chemistry (journal) , lipidology , nuclear magnetic resonance spectroscopy , sodium , chromatography , biochemistry , organic chemistry , physics , astronomy , artificial intelligence , computer science
A method is described wherein the resolution of 31 P nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh‐Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example.