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The effect of beta blocking drugs on lipid peroxidation in rat heart in vitro
Author(s) -
Jenkins R. R.,
Del Signore C. M.,
Sauer P.,
Skelly C.
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536137
Subject(s) - lipid peroxidation , atenolol , chemistry , pharmacology , propranolol , lipophilicity , liposome , thiobarbituric acid , antioxidant , metoprolol , biochemistry , endocrinology , medicine , blood pressure
Abstract Beta‐adrenergic receptor blocking drugs include a structurally related class of drugs that are employed clinically to treat a variety of cardiovascular disorders. Since these drugs exert additional nonspecific effects including membrane stabilization, representative samples including atenolol, dilevolol, labetolol, metoprolol and propranolol were studied to determine their influence on lipid peroxidation. Homogenates or liposomes of adult rat hearts were incubated in the presence of various concentrations of propranolol or equivalent concentrations of dilevolol, labetolol, metoprolol or atenolol. Lipid peroxidation was stimulated with 50 μM FeSO 4 , 5 μM t ‐butyl hydroperoxide (homogenates) or 0.2 mM citrate FeSO 4 (liposomes) plus O 2 . Lipid peroxidation, as assessed by both the thiobarbituric acid reaction and chemiluminescence, was reduced in a dose‐dependent manner as the propranolol concentration was increased from 1 to 10 mM. The five beta‐adrenergic receptor blocking drugs reduced lipid peroxidation both in crude homogenates and in liposomes; their effectiveness was related to their lipophilicity. Dilevolol, propranolol, labetolol and metoprolol at a concentration of 20 mM reduced lipid peroxidation by 45%, 37%, 35% and 28%, respectively. The hydrophilic blocker atenolol was ineffective in reducing lipid peroxidation event at elevated concentrations. Lipophilic beta‐blocking drugs apparently are capable of exerting an antioxidant effect in protecting membrane lipids against peroxidation.