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Specificity of soybean lipoxygenase‐1 in hydrated reverse micelles of sodium bis (2‐ethylhexyl)sulfosuccinate (aerosol OT)
Author(s) -
Shkarina Tatya.,
Kühn Hartmut,
Schewe Tankred
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536026
Subject(s) - micelle , chemistry , linoleic acid , chromatography , aqueous solution , lipoxygenase , pulmonary surfactant , arachidonic acid , organic chemistry , enzyme , fatty acid , biochemistry
Soybean lipoxygenase‐1 (EC 1.13.11.33) was purified by fast protein liquid chromatography on a MONO Q column and studied with respect to the conversion of linoleic and arachidonic acids in reverse micelles of sodium bis (2‐ethylhexyl)sulfosuccinate in n ‐octane. In this system the specific activities were lower by one order of magnitude than those in the corresponding aqueous system. High‐performance liquid chromatography analyses indicated the predominant formation of 13 S ‐hydroperoxy‐9 Z ,11 E ‐octadecadienoic acid (13‐HpODE) from linoleic acid and of 15 S ‐hydroperoxy‐5 Z ,8 Z ,11 Z ,13 E ‐eicosatetraenoic acid (15) from arachidonic acid both in the aqueous system and in the reverse micelles. After sedimentation of the hydrated reverse micelles by ultracentrifugation, both linoleic acid and 13‐HpODE were found to be enriched in the micelles with only small amounts of these compounds present in n ‐octane. It is proposed that substrates and products of the lipoxygenase reaction are located mainly in the surfactant shell of the hydrated reverse micelles and reach the micelle‐entrapped enzyme by diffusion into the aqueous interior space.