Premium
Studies on the transfer of tocopherol between lipoproteins
Author(s) -
Traber Maret G.,
Lane Jerome C.,
Lagmay Nora R.,
Kayden Herbert J.
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536020
Subject(s) - chemistry , tocopherol , very low density lipoprotein , lipidology , lipoprotein , in vivo , high density lipoprotein , clinical chemistry , chromatography , cholesterol , cholesterylester transfer protein , biochemistry , vitamin e , endocrinology , antioxidant , biology , microbiology and biotechnology
The net transfer of labeled α‐tocopherol from donor to acceptor lipoproteins at physiological concentrations was investigated. Labeled lipoproteins were isolated i) following in vitro addition of [3,4‐ 3 H] all rac ‐α‐tocopherol to plasma, or ii) from plasma obtained 12–16 h after ingestion by normal subjects of an oral dose (100 mg each) of 2 R ,4′ R ,8′ R ‐α‐[5,7‐(C 2 H 3 ) 2 ]tocopheryl acetate and 2 S ,4′ R′ ,R‐α‐[5‐C 2 H 3 ]tocopheryl acetate. A constant amount (on a protein basis) of labeled lipoprotein was incubated with an increasing amount of unlabeled acceptor lipoprotein for 2 h at 37°C. No discrimination between stereoisomers of α‐tocopherol was detected. Labeled VLDL and labeled LDL (very low and low density lipoproteins, respectively) tended to retain their labeled tocopherol. Labeled high density lipoproteins (HDL) readily transferred the labeled tocopherol to VLDL (>60% transferred), while the transfer to LDL was dependent upon the ratio of labeled HDL/LDL with a lower net transfer at higher ratios. This dependency of the distribution of tocopherol upon the ratio of HDL/LDL was also observed in vivo . The tocopherol/mg HDL protein was measured in 11 subjects with varying HDL levels. As the %HDL in the plasma increased from 14 to 50%, the tocopherol/HDL protein also increased (r 2 =0.37, P <0.05).