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Age‐related accumulation of phosphatidylcholine hydroperoxide in cultured human diploid cells and its prevention by α‐tocopherol
Author(s) -
Suzuki Toshihide,
Miyazawa Teruo,
Fujimoto Kenshiro,
Otsuka Miki,
Tsutsumi Masako
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02536004
Subject(s) - phosphatidylcholine , phospholipid , population , chemiluminescence , biology , biochemistry , tocopherol , wi 38 , in vitro , ploidy , antioxidant , chemistry , andrology , microbiology and biotechnology , vitamin e , chromatography , medicine , membrane , gene , demography , sociology
The levels of phosphatidylcholine hydroperoxide in serially cultured human fetal diploid fibroblasts at various population doubling levels were determined by high‐performance liquid chromatography combined with chemiluminescence detections. This methodology utilizes a mixture of cytochrome c and luminol as post‐column hydroperoxide group specific luminescent reagents. The cellular hydroperoxide content increased with age from 0.34 to 27.72 pmol/10 6 cells. At the end of the cells' in vitro lifespan (51st population doubling level), the hydroperoxide content per 10 6 cells reached about 80 times the level found in cells of the 20th population doubling level. Supplementation of exogenous α‐tocopherol to the culture medium prevented hydroperoxide accumulation, but did not extent the lifespan in vitro . The results indicate that substantial intracellular phospholipid hydroperoxide accumulation occurred in the course of aging of human fetal liploid fibroblasts.