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Double‐bond localization in heneicosapentaenoic acid by a gas chromatography/mass spectrometry (GC/MS) method
Author(s) -
Yamamoto Kohei,
Shibahara Akira,
Nakayama Takao,
Kajimoto Goro
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535982
Subject(s) - chemistry , double bond , mass spectrometry , gas chromatography–mass spectrometry , chromatography , gas chromatography , fragmentation (computing) , adduct , fatty acid , bond cleavage , derivatization , hydrazine (antidepressant) , organic chemistry , catalysis , computer science , operating system
Double‐bond locations in heneicosapentaenoic acid from eel lipids were determined by a method involving gas chromatography/mass spectrometry (GC/MS). The methyl esters of the pentaenoate fraction were first partially reduced with hydrazine. The resulting cis ‐monoenoates were then methylthiolated, and the resultant adducts were analyzed by GC/MS. The key fragmentation ions generated by the cleavage between the methylthio‐substituted carbons were used to ascertain the original double‐bond positions in the native fatty acid esters. Based on mass spectral evidence, the acid was identified as all‐ cis ‐6,9,12,15,18‐heneicosapentaenoic acid.