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Specific accumulation of docosahexaenoic acid (22∶6n−3) in brain lipids during development of juvenile turbot Scophthalmus maximus L.
Author(s) -
Mourente Gabriel,
Tocher Douglas R.,
Sargent John R.
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535970
Subject(s) - turbot , docosahexaenoic acid , galactolipids , polyunsaturated fatty acid , biology , scophthalmus , phosphatidylinositol , lipidology , fatty acid , zoology , phospholipid , biochemistry , dry matter , food science , medicine , endocrinology , fish <actinopterygii> , fishery , membrane , chloroplast , kinase , gene
The changes in the lipid class and the fatty acid compositions of total lipids and individual glycerophospholipids which occur in brain during development of juvenile turbot ( Scophthalmus maximus L.) were investigated. Fish were sampled during a 10‐week period immediately following weaning from a live feed to a pellet diet. During this period, brain dry weight increased over 6‐fold. The percentages of protein, cholesterol and galactolipids increased in brain during development, presumably reflecting increased membrane maturation and myelination processes. The percentages of docosahexaenoic acid (DHA; 22∶6n−3) were low at the beginning of the study period. However, DHA specifically accumulated in juvenile turbot brain during development. The percentages of DHA increased in total lipid, total diradyl glycerophosphocholine (GPC), total diradyl glycerophosphoethanolamine (GPE), phosphatidylserine (PS) and phosphatidylinositol (PI) reaching 26.1%, 25.8%, 40.8%, 47.1% and 17.9% of the total fatty acids, respectively, by the end of the 10‐week period. The percentages of other n−3 polyunsaturated fatty acids (PUFA) and n−6 PUFA generally decreased during this period, as did that of monoenes; the percentages of saturated fatty acids remained relatively constant. Non‐linear regression analysis showed that the increase in DHA in total lipid, GPC, GPE and PS fitted 1st order rate kinetics (plus offset) allowing maximum values for the percentages of DHA in each lipid class to be estimated.