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Compounds biologically similar to platelet activating factor are present in stored blood components
Author(s) -
Silliman Christopher C.,
Johnson Chris A.,
Clay Keith L.,
Thurman Gail W.,
Ambruso Daniel R.
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535939
Subject(s) - platelet activating factor , chemistry , biological activity , radioimmunoassay , polyclonal antibodies , platelet , whole blood , priming (agriculture) , receptor , antagonist , antibody , chromatography , biochemistry , endocrinology , biology , immunology , in vitro , botany , germination
Agents which prime the neutrophil NADPH oxidase develop during routine storae of whole blood and packed red blood cells. This plasma priming activity can be inhibited by bepafant (WEB 2170), a specific platelet activating factor (PAF) receptor antagonist. Quantitation of the priming agent(s), by a commercially available radioimmunoassay for PAF, reproducibly demonstrated high levels of PAF activity. However, analysis of these plasma samples from stored blood components by gas chromatography/mass spectroscopy did not reveal any 1‐ O ‐alkyl‐2‐acetyl‐ sn ‐glycero‐3‐phosphocholine. We conclude that the polyclonal antibody to PAF used in these studies may have recognized different epitopes of a family of heterogeneous, biologically active lipids that manifest their effects through the PFA receptor.