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High‐performance liquid chromatography of human milk triacylglycerols and gas chromatography of component fatty acids
Author(s) -
Dotson Karen D.,
Jerrell John P.,
Picciano Mary Frances,
Perkins Edward G.
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535876
Subject(s) - chemistry , chromatography , high performance liquid chromatography , gas chromatography , palmitic acid , oleic acid , acetone , fatty acid , organic chemistry , biochemistry
Human milk triacylglycerols were separated by high‐performance liquid chromatography. A 5‐μ Supelcosil LC‐18 column (Supelco, Inc., Bellefonte, PA) was used with acetone/acetonitrile (64∶36, vol/vol) as mobile phase. Triacylglycerols were tentatively identified based on theoretical carbon number and relative retention time. Despite changes resulting from dietary fat variation, the major component triacylglycerols were those composed of palmitic, oleic and linoleic acids. Triacylglycerols with palmitic, stearic and oleic acids were present as minor components. Fatty acids were quantified by gas chromatography relative to an internal standard. Ratios of n−6/n−3 fatty acids were found to be high than previously reported.