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The effect of a histidine‐excess diet on cholesterol synthesis and degradation in rats
Author(s) -
HitomiOhmura Eri,
Amano Nobuyuki,
Aoyama Yoritaka,
Yoshida Akira
Publication year - 1992
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535845
Subject(s) - medicine , cholesterol , endocrinology , clinical chemistry , lipidology , histidine , basal (medicine) , coenzyme a , biology , in vivo , chemistry , reductase , biochemistry , enzyme , insulin , microbiology and biotechnology
Feeding a diet high in excess histidine (5% L‐histidine) resulted in hypercholesterolemia and enlargement of the liver in rats. To clarify the mechanism of the hypercho‐lesterolemia cholesterol synthesis and degradation were followed. We found that hepatic 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase activity in histidine‐excess diet rats was significantly higher than in rats fed a basal diet. Incorporation of [ 3 H]water into cholesterol of liver slices from rats fed the histidine‐excess diet was higher than incorporation into liver slices from rats fed the basal diet (expressed per liver per 100 g body weight). In vivo incorporation of [ 3 H]water into hepatic cholesterol was also higher, but the incorporation into cholesterol of the small intestine was lower in histidine‐fed rats than in rats fed the basal diet (expressed per liver per 100 g body weight). Hepatic cholesterol 7α‐hydroxylase activity was similar in both groups. The data suggest that the hypercholester‐olemia caused by histidine‐excess diet appears to be due to the stimulation of cholesterol synthesis in the liver.