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A single‐step method for determination of the specific radioactivity of lipids
Author(s) -
Noble R. C.,
Shand J. H.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535838
Subject(s) - lipidology , clinical chemistry , chromatography , chemistry , radiochemistry , biochemistry
The use of a liquid scintillation counter to measure both the mass and the radioactivity content of charred 14 C‐labeled lipid bands from thin layer chromatoplates has been evaluated. Following lipid mass determination from a measurement of the external standard channels ratio, a suitable choice of counting parameters enabled a reproducible and efficient 14 C count to be obtained over virtually the whole range of lipid concentrations tested. Although the charring procedure resulted in some loss of radioactivity, the efficiency of counting remained high enough for accurate dpm measurements.