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Cholesterol and phospholipid composition of erythroblasts isolated from Mouse Spleen after Rauscher Leukemia virus infection
Author(s) -
Montfoort A.,
Boere W. A. M.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535819
Subject(s) - phosphatidylethanolamine , phospholipid , phosphatidylcholine , sphingomyelin , phosphatidylserine , arachidonic acid , cholesterol , biochemistry , choline , lecithin , linoleic acid , phosphatidylinositol , chemistry , biology , plasmalogen , spleen , fatty acid , microbiology and biotechnology , immunology , membrane , kinase , enzyme
Erythroblasts (more than 95% pure) were isolated from MuLV‐R infected mouse spleen. The cholesterol, phospholipid, and fatty acid composition of the diacyl and plasma logen derivatives of the choline and ethanolamine phosphoglycerides of mouse erythroblasts, red blood cells enriched with reticulocytes, and mature erythrocytes have been compared. It was demonstrated that during maturation of the mouse red blood cells, the following relative changes in the lipid composition occur: (a) The cholesterol/phospholipid molar ratio increases from 0.35 to 0.77 (b) The sphingomyelin concentration increases from 3.0 to 12.1 and the phosphatidylserine concentration from 5.3 to 11.2 as percentage of the total phospholipid. (c) Phosphatidylcholine decreases from 52.0 to 46.2% and phosphatidylinositol from 6.7 to 3.1%, respectively. (d) The percentage of linoleic acid in the phosphatidylcholine fraction increases more than two fold and is predominantly found at the 2‐position. However, molecules containing linoleic acid at the 1‐position are found to be more abundant in erythroblasts than in mature erythrocytes. (e) Phosphatidylethanolamine has been found to contain a high percentage of arachidonic acid, which was mainly found at the 2‐position. The percentage of arachidonic acid at the 1‐position increases during maturation, while the percentage at the 2‐position decreses. (f) The plasmalogen derivatives of the choline phosphoglycerides were found to be almost absent in mouse red blood cells (<0.5%), while the ethanolamine plasmalogen phosphoglycerides represented about 8% of the phospholipids.