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Fatty acid synthesis from [2‐ 14 C]acetate in normal and peroxisome‐deficient (Zellweger) fibroblasts
Author(s) -
Poulos A.,
Usher S.,
Paton B. C.
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535771
Subject(s) - zellweger syndrome , peroxisome , clinical chemistry , lipidology , fatty acid , biochemistry , peroxisome proliferator , chemistry , plasmalogen , medicine , endocrinology , biology , membrane , receptor , phospholipid
The incorporation of [2‐ 14 C]acetate into the lipids of normal and peroxisome‐deficient (Zellweger's syndrome) skin fibroblasts was examined. Most of the label was incorporated into triacyglycerol fatty acids in normal as well as Zellweger's syndrome cells. Triacylglycerols and cholesteryl esters in Zellweger's syndrome cells contained increased levels of labelled saturated and monounsaturated very long‐chain fatty acids (VLCFA, that is fatty acids with more than 22 carbon atoms), in particular hexacosanoic (26∶0) and hexacosaenoic (26∶1) acids. As traces of labelled VLCFA with up to 32 carbon atoms were detected in triacylglycerols even in control cells it is probable that these fatty acids are formed naturally during the elongation process. Our data suggest that peroxisomes are involved in the chain shortening of the saturated and monounsaturated VLCFA.