Premium
Turnover of fatty acyl‐CoA oxidase in the liver of rats fed on a partially hydrogenated marine oil
Author(s) -
Horie Shuchi,
Suga Tetsuya
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535764
Subject(s) - peroxisome , clinical chemistry , lipidology , carnitine , oxidase test , chemistry , enzyme , carnitine palmitoyltransferase i , biochemistry , medicine , endocrinology , beta oxidation , biology , receptor
The change in turnover of fatty acyl‐CoA oxidase (FAO), the rate‐limiting enzyme of the peroxisomal β‐oxidation system, was investigated in rats fed a 30% (w/w) partially hydrogenated marine oil (PHMO) diet. The FAO activity increased five‐fold after two weeks of PHMO feeding, and decreased after withdrawal of the diet. Based on in vivo experiments using L‐[4,5‐ 3 H]leucine and an immunoprecipitation technique, the increase in the activity of FAO could be accounted for by a 1.6‐fold higher rate of FAO synthesis and a 3.4‐fold slower rate of FAO degradation as compared to controls. In the same PHMO‐fed rats, the rates of synthesis and degradation of carnitine palmitoyltransferase were 1.8‐fold higher and 2.0‐fold slower, respectively, as compared to controls. The results indicate that the observed increase in the activity of the enzymes of peroxisomal β‐oxidation is mainly due to a reduced rate of FAO degradation in the liver of rats fed the PHMO diet.