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( 3 H/ 14 C)Beta‐methylheptadecanoic acid subcellular distribution and lipid incorporation in mouse heart
Author(s) -
Livni Eli,
Ito Susumu,
Kassis Amin I.,
Elmaleh David R.
Publication year - 1990
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535755
Subject(s) - lipidology , clinical chemistry , catabolism , chemistry , biochemistry , lipid droplet , fatty acid , mitochondrion , beta (programming language) , lipid metabolism , distribution (mathematics) , thin layer chromatography , metabolism , chromatography , mathematical analysis , mathematics , computer science , programming language
Beta‐methylfatty acids are transported into myocardial cells as other fatty acids would be, but are incapable, for example, of undergoing complete catabolism. Our previous studies have established the structure‐location relationship and the imaging characteristics of these analogs. In the present study in mouse myocardium, microautoradiography and electron microscopy were used to show the distribution of [7,8‐ 3 H] beta‐methylheptadecanoic acid ([ 3 H]BMHA) in mitochondria and lipid droplets. Thin‐layer chromatography demonstrated the presence of [1‐ 14 C] beta‐methylheptadecanoic acid ([ 14 C]BMHA) and its metabolites in various lipid pools. These studies complement our earlier findings which showed that similarities exist in the initial metabolic fate of BMHA and physiological fatty acids.