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Stimulation of fatty acid synthesis by 4β‐phorbol‐12‐myristate‐13‐acetate in isolated rat hepatocytes
Author(s) -
Nomura Takahide,
Tachibana Masakatsu,
Nomura Hiroko,
Hagino Yasumichi
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535703
Subject(s) - clinical chemistry , phorbol , stimulation , protein kinase c , fatty acid synthesis , lipidology , fatty acid , extracellular , phorbol ester , chemistry , biochemistry , medicine , endocrinology , biology , kinase
The tumor‐promoting agent 4β‐phorbol‐12‐myristate‐13‐acetate (TPA) is shown to be a potent stimulator of fatty acid synthesis in isolated rat hepatocytes. The maximal effect of TPA is seen at 10 −6 M, and the concentration for half‐maximal effect is ca. 10 −8 M. Stimulation of fatty acid synthesis by TPA is shown not to require the presence of extracellular Ca ++ . TPA produces a significant increase in lactate and pyruvate accumulation. The possible involvement of protein kinase C in short‐term regulation of fatty acid synthesis in the liver is discussed.