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Quantitative analysis of polyenoic phospholipid molecular species by high performance liquid chromatography
Author(s) -
Takamura Hitoshi,
Narita Hiroshi,
Urade Reiko,
Kito Makoto
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535701
Subject(s) - phospholipid , phosphatidylethanolamine , high performance liquid chromatography , chromatography , chinese hamster , chemistry , solvent , phosphatidylcholine , biochemistry , membrane , dna
Abstract The quantitative analysis of phospholipid molecular species containing polyenoic fatty acids is described. Dinitrobenzoyl derivatives of diacylglycerols prepared from phospholipids were separated into individual molecular species by reversed‐phase high performance liquid chromatography (HPLC) using a combination of two solvent systems and were quantified at 254 nm. Thirty‐six molecular species were resolved from the phosphatidylcholines of rat hearts, human platelets and Chinese hamster V79‐R cells. The derivatives of alkenylacyl molecular species from platelet phosphatidylethanolamine were resolved concomitantly with diacyl molecular species.