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Thiobarbituric acid reaction of methyl arachidonate monohydroperoxide isomers
Author(s) -
Terao J.,
Matsushita S.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535681
Subject(s) - chemistry , ferrous , thiobarbituric acid , yield (engineering) , sulfate , chromatography , arachidonic acid , methylene blue , gas chromatography , organic chemistry , lipid peroxidation , antioxidant , catalysis , materials science , enzyme , photocatalysis , metallurgy
Methyl ester of monohydroperoxy eicosatetraenoic acid (MeHPETE) was prepared from methylene blue sensitized photooxidation products of methyl arachidonate. The thiobarbituric acid (TBA) value of MeHPETE was increased by adding ferrous sulfate to the reaction mixture. A linear relationship existed betweent he TBA value and the concentration of MeHPETE when ferrous sulfate was added. By using high performance liquid chromatography, MeHPETE was separated into 5 fractions whose isomeric compositions were determined by gas chromatography‐mass spectrometry. The results of the TBA test for each fraction suggest that all of the MeHPETE isomers are positive to the TBA test. It is concluded that each isomer of HPETE formed by peroxidation of arachidonic acid in a biological system can yield TBA‐reacting materials during the test reaction.