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cis ‐9,10‐Epoxystearic acid in human leukocytes: Isolation and quantitative determination
Author(s) -
Fahistadius Per
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535645
Subject(s) - chromatography , chemistry , clinical chemistry , lipidology , quantitative analysis (chemistry) , biochemistry
Racemic cis ‐9,10‐epoxystearic acid was isolated from total lipids of human leukocytes. Identification of the epoxy acid was based mainly on gas liquid chromatographicmass spectrometric analysis and on its chemical conversion into threo ‐9,10‐dihydroxystearic acid. A mass spectrometric method for quantitative determination of cis ‐9,10‐epoxystearic acid using the tetradeuterated compound as internal standard was developed. Using this method, nonstimulated human leukocytes were found to contain 5.1±2.2 μg (SD) of cis ‐9,10‐epoxystearic acid per 10 9 cells (n=8). More than 90% of the epoxy acid occurred in its esterified form in leukocyte lipids.