Premium
Cyclic AMP increases incorporation of exogenous fatty acids into triacylglycerols in hamster fibroblasts
Author(s) -
Mazière Cécile,
Mazière JeanClaude,
Mora Liliana,
Auclair Martine,
Polonovski Jacques
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535641
Subject(s) - hamster , chemistry , arachidonic acid , diacylglycerol kinase , biochemistry , phosphatase , medicine , phosphorylation , endocrinology , biology , enzyme , protein kinase c
Incorporation of various exogenous saturated or unsaturated [ 14 C]labeled fatty acids (palmitic, stearic, oleic, linoleic and arachidonic) into triacylglycerols by hamster fibroblasts was markedly enhanced (two‐to fourfold) in the presence of theophylline or dibutyryl cyclic adenosinemonophosphate (dbcAMP). This effect was observed for short‐term (1–6 hr) as well as long‐term (15–24 hr) preincubation with dbcAMP. In the presence of sodium fluoride, a phosphoprotein phosphatase inhibitor, measurement of diacylglycerol acyltransferase (DGAT) activity in cells pretreated with dbcAMP pointed out a marked increase (3X) in specific activity. The results suggest that DGAT activity in fibroblasts could be activated by a cAMP‐dependent phosphorylation process.